A specialized installation has been developed for microscopic study of the Reactive Oxygen Species (ROS) formation during microwave irradiation of biological samples with automated control / mechanized tube and real-time data acquisition. The above installation can be used in biomedical practice for: standardization or certification of the microwave sources; testing of the potential antioxidants that protect tissues from ROS-induced effects; testing fluorescent sensors for ROS; analysis of ROS localization and distribution in various tissues in order to establish specific pharmaco-physiotherapeutic and toxicological localizations of ROS in different topographic-anatomical zones. The paper pays special attention to the singlet oxygen produced by the samples upon microwave treatment, as a physiologically active and highly reactive agent.
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He YY, Council SE, Feng L, Bonini MG, Chignell CF. Spatial distribution of protein damage by singlet oxygen in keratinocytes. Photochem Photobiol. 2008;84(1):69-74. Available from: https://doi.org/10.1111/j.1751-1097.2007.00199.x
Di Mascio P, Kaiser SP, Devasagayam TP, Sies H. Biological significance of active oxygen species: in vitro studies on singlet oxygen-induced DNA damage and on the singlet oxygen quenching ability of carotenoids, tocopherols and thiols. Adv Exp Med Biol. 1991;283:71-7. Available from: https://doi.org/10.1007/978-1-4684-5877-0_7
Ray RS, Mujtaba SF, Dwivedi A, Yadav N, Verma A, Kushwaha HN, et al. Singlet oxygen mediated DNA damage induced phototoxicity by ketoprofen resulting in mitochondrial depolarization and lysosomal destabilization. Toxicology. 2013;314(2-3):229-37. Available from: https://doi.org/10.1016/j.tox.2013.10.002
Fenoglio I, Ponti J, Alloa E, Ghiazza M, Corazzari I, Capomaccio R, et al. Singlet oxygen plays a key role in the toxicity and DNA damage caused by nanometric TiO₂ in human keratinocytes. Nanoscale. 2013;5(14):6567-76. Available from: https://doi.org/10.1039/c3nr01191g
Rokitskaya TI, Kotova EA, Agapov II, Moisenovich MM, Antonenko YN. Unsaturated lipids protect the integral membrane peptide gramicidin A from singlet oxygen. FEBS Lett. 2014;588(9):1590-5. Available from: https://doi.org/10.1016/j.febslet.2014.02.046
Zarębski M, Kordon M, Dobrucki JW. Photosensitized damage inflicted on plasma membranes of live cells by an extracellular generator of singlet oxygen--a linear dependence of a lethal dose on light intensity. Photochem Photobiol. 2014;90(3):709-15. Available from: https://doi.org/10.1111/php.12216
Savin YV, Goryachev LV, Adamenkov YA, Rakhimova TV, Mankelevich YA, Popov NA, et al. Singlet oxygen production and quenching mechanisms in travelling microwave discharges. J Phys D Appl Phys. 2004;37(22):3121-8. Available from: http://dx.doi.org/10.1088/0022-3727/37/22/010
Popović S, Rašković M, Kuo SP, Vušković L. Reactive oxygen emission from microwave discharge plasmas. J Phys Conf Ser. 2007;86:012013. Available from: http://dx.doi.org/10.1088/1742-6596/86/1/012013
Pitz GA, Lange MA, Perram GP. Singlet oxygen kinetics in a double microwave discharge. In: High-Power Laser Ablation V. 2004;5448:1039-48. Available from: https://doi.org/10.1117/12.548707
Yu Y, Zhang T, Zheng L, Yu J. Detection of reactive oxygen species generated by microwave electrodeless discharge lamp and application in photodegradation of H₂ Korean J Chem Eng. 2013;30:1423-8. Available from: https://doi.org/10.1007/s11814-013-0074-z
Lange MA, Pitz GA, Perram GP. Effect of residence time on singlet oxygen production in microwave and RF discharges. In: AIP Conf Proc. 2010;1278:482-91. Available from: https://doi.org/10.1063/1.3507137
Özorak A, Nazıroğlu M, Çelik Ö, Yüksel M, Özçelik D, Özkaya MO, et al. Wi-Fi (2.45 GHz)- and mobile phone (900 and 1800 MHz)-induced risks on oxidative stress and elements in kidney and testis of rats during pregnancy and the development of offspring. Biol Trace Elem Res. 2013;156(1-3):221-9. Available from: https://doi.org/10.1007/s12011-013-9836-z
Saygin M, Caliskan S, Karahan N, Koyu A, Gumral N, Uguz A. Testicular apoptosis and histopathological changes induced by a 2.45 GHz electromagnetic field. Toxicol Ind Health. 2011;27(5):455-63. Available from: https://doi.org/10.1177/0748233710389851
Gumral N, Naziroglu M, Koyu A, Ongel K, Celik O, Saygin M, et al. Effects of selenium and L-carnitine on oxidative stress in blood of rat induced by 2.45-GHz radiation from wireless devices. Biol Trace Elem Res. 2009;132(1-3):153-63. Available from: https://doi.org/10.1007/s12011-009-8372-3
Türker Y, Nazıroğlu M, Gümral N, Celik O, Saygın M, Cömlekçi S, et al. Selenium and L-carnitine reduce oxidative stress in the heart of rat induced by 2.45-GHz radiation from wireless devices. Biol Trace Elem Res. 2011;143(3):1640-50. Available from: https://doi.org/10.1007/s12011-011-8994-0
Gradov OV. Experimental setups for ozonometric microscopy. Biomed Eng. 2013;46(6):260-4.
Gradov OV. [Experimental setups for ozonometric microscopy]. Med Tekh. 2012;(6):42-7. Russian. PMID: 23304991. Available from: https://doi.org/10.1007/s10527-013-9319-8
Yin G, Liao PH, Lo KV. An ozone/hydrogen peroxide/microwave-enhanced advanced oxidation process for sewage sludge treatment. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2007;42(8):1177-81. Available from: https://doi.org/10.1080/10934520701418706
Wong WT, Chan WI, Liao PH, Lo KV. A hydrogen peroxide/microwave advanced oxidation process for sewage sludge treatment. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2006;41(11):2623-33. Available from: https://doi.org/10.1080/10934520600928086
Zmyślony M, Politanski P, Rajkowska E, Szymczak W, Jajte J. Acute exposure to 930 MHz CW electromagnetic radiation in vitro affects reactive oxygen species level in rat lymphocytes treated by iron ions. Bioelectromagnetics. 2004;25(5):324-8. Available from: https://doi.org/10.1002/bem.10191
Kesari KK, Kumar S, Behari J. 900-MHz microwave radiation promotes oxidation in rat brain. Electromagn Biol Med. 2011;30(4):219-34. Available from: https://doi.org/10.3109/15368378.2011.587930
Ni S, Yu Y, Zhang Y, Wu W, Lai K, Yao K. Study of oxidative stress in human lens epithelial cells exposed to 1.8 GHz radiofrequency fields. PLoS One. 2013;8(8):e72370. Available from: https://doi.org/10.1371/journal.pone.0072370
Brescia F, Sarti M, Massa R, Calabrese ML, Sannino A, Scarfì MR. Reactive oxygen species formation is not enhanced by exposure to UMTS 1950 MHz radiation and co-exposure to ferrous ions in Jurkat cells. Bioelectromagnetics. 2009;30(7):525-35. Available from: https://doi.org/10.1002/bem.20502
Campisi A, Gulino M, Acquaviva R, Bellia P, Raciti G, Grasso R, et al. Reactive oxygen species levels and DNA fragmentation on astrocytes in primary culture after acute exposure to low intensity microwave electromagnetic field. Neurosci Lett. 2010;473(1):52-5. Available from: https://doi.org/10.1016/j.neulet.2010.02.018
Yao K, Wu W, Wang K, Ni S, Ye P, Yu Y, et al. Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells. Mol Vis. 2008;14:964-9. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC2391079/
Marjanović AM, Pavičić I, Trošić I. Biological indicators in response to radiofrequency/microwave exposure. Arh Hig Rada Toksikol. 2012;63(3):407-16. Available from: https://doi.org/10.2478/10004-1254-63-2012-2215
Mishina NM, Tyurin-Kuzmin PA, Markvicheva KN, Vorotnikov AV, Tkachuk VA, Laketa V, et al. Does cellular hydrogen peroxide diffuse or act locally? Antioxid Redox Signal. 2011;14(1):1-7. Available from: https://doi.org/10.1089/ars.2010.3539
Gradov OV, Jablokov AG. Novel morphometrics-on-a-chip: CCD- or CMOS-lab-on-a-chip based on discrete converters of different physical and chemical parameters of histological samples into the optical signals with positional sensitivity for morphometry of non-optical patterns. J Biomed Technol. 2016;(2):1-29. Available from: http://dx.doi.org/10.15393/j6.art.2016.3642
Gradov OV, Gradova MA. Microwave enthrakometric labs-on-a-chip and on-chip enthrakometric catalymetry: from non-conventional chemotronics towards microwave-assisted chemosensors. Chemosensors. 2019;7(4):48. Available from: https://doi.org/10.3390/chemosensors7040048
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